周宁①  黎冬梅①  叶家卫②

【摘要】目的：评价幽门螺杆菌粪便抗原（HpSA)检测诊断幽门螺杆菌感染的特异性和敏感性。方法：采用酶联免疫分析法检测150例反复腹痛或伴有恶心、呕吐等上消化道症状患儿的幽门螺杆菌粪便抗原，同时以¹³C尿素呼气试验(¹³C-UBT)检查作对照，对HpSA酶联免疫 法与¹³C尿素呼气试验(¹³C-UBT)检查的阳性结果进行比较。结果：150例患儿中，HpSA酶联免疫法阳性率30%,对照法阳性率为29.33%, 两法阳性检测率比较差异无统计学意义，HpSA酶联免疫法与对照法比较其敏感度和特异度分别为84.09%和92.45%。结论：HpSA酶联免疫 法是一种操作简便、安全、可靠的诊断幽门螺杆菌感染的方法，尤其适用于儿童幽门螺杆菌感染的普查。

【关键词】幽门螺杆菌；粪便抗原；酶联免疫法

Clinical Application of Helicobacter Pylori Feces Antigen Detection/ZHOU Ning,LI Dong-mei,YE Jia-wei.//Medical Innovation of China, 2012, 9(14):084-085

【Abstracl】 Objective:To evaluation specificity and sensitivity of Helicobacter pylori stool antigen (HpSA) detection in diagnosis of Helicobacter pylori infection.Methods:Enzyme-linked immunosorbent assay of 150 cases of recurrent abdominal pain or nausea, vomiting and other gastrointestinal symptoms in children on the Helicobacter pylori stool antigen, while ¹³C urea breath test (¹³C-UBT) for the control check, the HpSA enzyme- linked immunoassay and ¹³C urea breath test (¹³C-UBT)-positive results were compared to evaluate the HpSA diagnostic sensitivity and specificity of Helicobacter pylori infection. Results:150 cases in children, HpSA ELISA-positive rate of 30%, the positive rate was 29.33% control method, the positive detection rate of these two methods was no significant statistical difference, HpSA ELISA method were compared with the sensitivity and specificity degrees were 84.09% and 92.45%.Conclusion:HPSA ELISA is a simple, safe and reliable method of diagnosis of Helicobacter pylori infection, especially for Helicobacter pylori infection in the census.

【Key words】 Helicobacter pylori; Stool antigen ； Enzyme-linked immunosorbent assay

First-author's address:Maternal and Child Hospital of Haizhu District in Guang Zhou, Guangzhou 510240, China

doi ： 10.3969/j.issn.1674-4985.2012.14.054