摘要：目的  探讨时间分辨荧光法(TRFIA)、化学发光免疫分析法(CLIA)和酶联免疫吸附法(ELISA)检测胃蛋白酶原I(PGI)的相关性，及其临床实用性。方法在TRFIA法检测的临床标本中随机选择88份，分别用CLIA法和ELISA法再次检测PGI，分析三种方法检测结果的相关性；对体检人群、消化科门诊患者和住院患者进行回顾性分析，验证TR-FIA法检测PGI对胃病筛查的可行性。结果TRFIA法(Y)与CLIA法(X)，ELISA法(X)PGI检测结果均呈显著正相关(r分别为0.9928和0.9950，P<0.05，三种方法检测结果的相对偏差(SE%)均<10%；体检人群、消化科门诊患者、消化科住院患者的血清PGI水平分别为156.0±63.8ng/ml，197.8±113.8ng/ml，269.5±156.5ng/ml,三者差异具有统计学显著性意义(P<0.05)。结论TRFIA法检测PGI结果可靠、操作方便，未来可能用于胃病患者的筛查或辅助诊断。
关键词：胃蛋白酶原I；时间分辨荧光法；酶联免疫吸附法
中图分类号：R446. 112 文献标志码：A 文章编号：1671-7414(2014)01-132-04
doi：10. 3969/j. issn. 1671-7414. 2014. 01. 047

Abstract：Objective To evaluate the correlation of three pepsinogen I (PG I) detection methods and its clinical applications. Methods 88 serum samples were randomly selected, and PG I was detected by using time-resolved fluoroimmunoassay (TRFIA) ,chemiluminescence immunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA),respectively. Then, their results were processed using regression and correlation analysis. To verify the feasibility of gastric disease screening by PGI detection,3 575 people were divided into three groups：gastroenterology outpatients,gastroenterology hovSpitalized pa¬tients, and physical examination population. Results It showed a significant positive correlation between any two of the three methods (P<0. 05) ,and the detection values of the medical decision concentration by the three methods have good correla- tivity and can be converted to each other. PG I serum levels were 156. 0 + 63. 8 ng/ml, 197. 8 + 113. 8 ng/mh and 269. 5 d= 156. 5 ng/ml in outpatientvS, hospitalized patients, and physical examination population, respectively. There was significantly different between the three groups (P<0. 05). Conclusion PG I detection by TRFIA is simple and effective method. It may be used for the screening or auxiliary diagnosis of gastric disease in the future.
Keywords：pepsinogen I；time-resolved fluoroimmunoassay；enzyme-linked immunosorbent assay